Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Papio sp. |
Protein Variants | Comment | Organism |
---|---|---|
synthesis | expression of baboon uricase gene attached with Trx and hexahistidine tags in Escherichia coli Rosetta (DE3). The final yield of mature baboon uricase is 136.0 mg/l with enzyme activity of 17.93 U/mg | Papio sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
Urate | pH 8.2, 37°C | Papio sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Papio sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
urate + O2 + H2O | - |
Papio sp. | 5-hydroxyisourate + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Papio sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Papio sp. |